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Effect of Rho kinase inhibitor on S1P-stimulated cell migration. Fibroblast chemotaxis was performed to fibronectin (20 μg/ml) with varying concentrations of Y27632 added to the cells in the top wells. Vertical axis: migrated cell number per five high-power fields expressed as percentage of control (20 μg/ml HFn only). Horizontal axis: S1P concentration. Open bars, control; striped bars, 2 μM Y27632; hatched bars, 5 μM Y27632; solid bars, 10 μM Y27632. *P < 0.01 by two-way ANOVA.
