TABLE 1.

EFFECT OF PHARMACOLOGIC INHIBITORS ON S1P-AUGMENTED FIBROBLAST CHEMOTAXIS

	Control		S1P (10 μM)
	−	+	−	+
PTX (1 μg/ml)	39 ± 2	52 ± 2	340 ± 21	389 ± 20
Calphostin C (0.1 μM)	165 ± 6	78 ± 6*	687 ± 51	447 ± 31*
Y294002 (50 μM)	114 ± 5	29 ± 2*	328 ± 34	295 ± 13
PP2 (50 μM)	157 ± 5	80 ± 5*	341 ± 17	389 ± 12

Definition of abbreviations: Calphostin C, PKC inhibitor; PP2, Src kinase inhibitor; PTX, pertussis toxin; S1P, sphingosine 1-phosphate; Y294002, PI3-kinase inhibitor.

^*P < 0.05 by Student's t test compared with control.