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Micromolar levels of the ADO analog NECA are required to reprise ASL volume homoestasis. (A) XZ confocal images of ASL labeled with FITC-dextran (red) 8 hours after addition of PBS containing ADA and NECA. (B) Mean data taken from A. Squares, control; circles, ADA and 10−8 NECA; triangles, ADA and 10−7 M NECA; diamonds, ADA and 10−6 M NECA. All n = 7. *P < 0.05 to t = 0. †P < 0.05 to control samples at that time point. Scale bar is 7 μm.
