Figure 4.

Zinc (Zn) levels in the bronchoalveolar lavage and alveolar macrophages from wild-type and HIV-1–transgenic rats. Zinc was measured by a Zn-specific dye, FluoZin-3 (Invitrogen). Soluble form of this dye was used to measure Zn in the lavage (A), as described in Materials and Methods. (B and C) Flow cytometric determination of intracellular Zn by using a membrane-permeable form of FluoZin-3AM. Each value in A–C represents the mean ± SEM of six to eight rats in each group. *P < 0.05 compared to wild-type rats. Both HIV-1–transgenic and wild-type rats used in these experiments were 8 to 9 months old.