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. 2008 Sep 22;182(6):1073–1082. doi: 10.1083/jcb.200710188

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© 2008 Kim et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

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Figure 1. — Endocytosis of Fz7 and Dsh occurs in X. laevis CE movements. Embryos were microinjected into the two dorsal blastomeres at the four-cell stage with a combination of the indicated mRNAs (500 pg GFP Dsh, 500 pg myc Fz7, 500 pg YFP Wnt11, 500 pg myc Rab5, 500 pg GFP Rab5, 1 ng xβarr2-KRK/Q, 10 ng xβarr2 MO, and 10 ng Co MO). DMZ explants were dissected at stage 11–11.5 and then subjected to fluorescence analysis. (A) Dsh localized to punctate structures that colocalize with β2 adaptin in DMZ tissues. (B and C) Fz7 and Wnt11 also overlapped with Rab5. (D and E) Functional knockdown of βarr2 by xβarr2 MO and xβarr2-KRK/Q relocalized Dsh to the cell membrane and inhibited colocalization with β2 adaptin in cytoplasm. Bars, 20 μm.