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. 2008 Sep 22;182(6):1127–1139. doi: 10.1083/jcb.200712091

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© 2008 Hou et al.

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Figure 4. — The effector caspase Dcp-1 is not only required for nutrient starvation–induced autophagy but is also sufficient for the induction of autophagy during D. melanogaster oogenesis. (A) Germaria of the nutrient-deprived Dcp-1^Prev flies showed a dramatic decrease in LTR staining compared with nutrient-deprived wild-type flies shown in Fig. 3 B. DAPI staining of nuclei is shown in blue. (B) Degenerating stage 8 egg chambers (arrows) of nutrient-deprived Dcp-1^Prev flies showed a dramatic decrease in LTR staining compared with nutrient-deprived wild-type flies shown in Fig. 3 C. (C) Lack of Dcp-1 function (UASp-GFP-LC3 Dcp-1^Prev/Dcp-1^Prev; nanos-GAL4/+) resulted in uniform diffuse staining of GFP-LC3 rather than the punctate pattern observed in wild-type degenerating stage 8 egg chambers shown in Fig 3 C. (D) Dying egg chambers (arrows) of NGT/+; nanos-GAL4/UASp-fl-Dcp-1 flies that were conditioned on yeast paste showed a significant increase in punctate LTR staining (red) compared with healthy egg chambers (arrowheads). (E) Expression of activated Dcp-1 (a truncated form) and GFP-LC3 in the germ line (UASp-GFP-LC3/+; nanos-GAL4/nanos-GAL4 UASp-tDcp-1) resulted in abundant degenerating stage 8 egg chambers (arrows) with numerous GFP-LC3 puncta (green). DAPI staining of nuclei is shown in blue. Bars: (A) 20 μm; (B–E) 50 μm.