TABLE 1.
Constructs and primers used for PCRs
| Construct | Template | Primer | Sequence of primer |
|---|---|---|---|
| Mot1/pr | TCV satC | P10 | GGGATAACTAAGGGTTTCATACGTTACTACATCCCAGACCCT |
| P11 | TAATACGACTCACTATAGGGCTGCCGCCGTTTTTGG | ||
| Mot1d10/pr | TCV satC | P12 | AAGGGTTTCATACGTTACTACATCCC |
| P10 | TAATACGACTCACTATAGGGCTGCCGCCGTTTTTGG | ||
| R3(−)/pr11 | DI-72 | 23 | GTAATACGACTCACTATAGGGACCCAACAAGAGTAACCTG |
| 253 | TTGGAAATTCTCCTTAGCGAGTAAGACAGACTC | ||
| AU1/pr11 | PN-R′+AU1 | 248 | GTAATACGACTCACTATAGGAGACCCTGTCCAGGTAG |
| 251 | TTGGAAATTCTCCTTGTGCTCGAGTTGGATCC | ||
| GC1/pr11 | PN-GC1 | 248 | GTAATACGACTCACTATAGGAGACCCTGTCCAGGTAG |
| 251 | TTGGAAATTCTCCTTGTGCTCGAGTTGGATCC | ||
| R3(−)/art | DI-72 | 116 | GTAATACGACTCACTATAGGACACCTAACTTTCGT |
| 112 | TCGTCTTATTGGACGAGATAGTCACTTGACTAC | ||
| AU1/art | PN-R′+AU1 | 248 | GTAATACGACTCACTATAGGAGACCCTGTCCAGGTAG |
| 249 | TCGTCTTATTGGACGAGTGCTCGAGTTGGATCC |