Table 1.
RDCs measurements on lactose bound to Galectin-3.
| Free | DOGS-NTA Ammonium | DOGS-NTA Nickel salt | ||||
|---|---|---|---|---|---|---|
| RDC(Hz) | RDC(Hz) | RDC(Hz) | RDCbound(Hz)a | RDCcal(Hz)b | ||
| Glc(α) | C1-H1 | −4 | −3.9 | −4.5 | −14 | −13 |
| C2-H2 | 2.7 | 2.5 | 0.7 | −37 | −21 | |
| C3-H3 | 3.7 | 3.4 | 2.9 | −12 | −22 | |
| C5-H5 | 3.9 | 3.2 | 2.8 | −18 | −26 | |
| H1-H2 | −0.1 | −1.5 | −29 | −24 | ||
| Gal | C1-H1 | 1.7 | 1.5 | −2.6 | −84 | −86 |
| C2-H2 | 1.9 | 2.1 | −1.8 | −72 | −70 | |
| C3-H3 | 2.4 | 1.8 | −2.3 | −91 | −96 | |
| C4-H4 | 0.1 | 1 | 1.4 | 26 | 23 | |
| C5-H5 | 2.8 | 2.4 | −1.8 | −89 | −88 | |
| H1-H2 | 0.2 | 0.9 | 15 | 13 | ||
|
| ||||||
| Order tensor | Sxx | Syy | Szz | |||
|
| ||||||
| Free lactose | −1.1E-04 | −1.7E-04 | 1.8E-04 | |||
|
| ||||||
| Bound -lactose | −2.8E-04 | −2.9E-03 | 3.2E-03 | |||
a
RDCfor the bound state have been extracted using a dissociation constant of 1mM.
b
RDCs have been back-calculated using the lactose geometry found in the crystal structure, 1ULC.