FIG. 3.

Expression of ICP0 or β-Gal mRNA at 2 h p.i. RSC monolayers were infected at an MOI of 5, and total cellular RNA was harvested from triplicate cultures at 2, 4, and 6 h p.i. Total RNA was electrophoresed, blotted, transferred, and hybridized to probes specific for β-Gal, ICP0, or the cellular transcript L32, which was employed as a loading control. The signals were quantified with a PhosphorImager using ImageQuant software and normalized to the L32 control signal. The bar graphs represent the percentages of normalized signal relative to the 17-0pZ562gC signal, which was set to 100%.