TABLE 1.

Detection of ICP0 protein and ICP0 promoter activity during HSV reactivation in vivo^a

Strain	No. (%) of reactivating neurons containing indicated precipitate(s)/ total no. of neurons detected			No. (%) of ganglia containing one or more neurons positive for either viral protein or β-Gal/total no. of ganglia examined
	Blue	Blue + brown	Brown
17-0pZ562gJb	45/113 (40)	68/113 (60)	0/113 (0)	24/29 (83)
17-0pZ562gJc	26/77 (34)	51/77 (66)	0/77 (0)	22/26 (85)
17AH-0pZ562gJc	1/14 (7)	13/14 (93)	0/14 (0)	7/24 (29)

^aMice were infected with strain 17-0pZ562gJ and the corresponding LAT null mutant 17AH-0pZ562gJ, maintained for at least 40 days p.i., and subjected to the HS in vivo reactivation procedure. At 23 h poststress, ganglia were removed and processed to detect viral protein (brown precipitate) and promoter reporter activity (β-Gal; blue precipitate).

^bExperiment 1.

^cExperiment 2.