Figure 5.

Effect of box D point mutations on the nucleolar localization of U3 snoRNA. Fluorescently labeled wild-type (U3) and variant U3 RNAs with single-nucleotide changes in the box D element (ΔD-C212B, -U213G, -G214B, and -A215U) were injected into oocytes, and nuclear spreads were made 1 h after injection. The particular nucleotides in box D (CUGA) that were altered are indicated on the right. The DIC images show two to three nucleoli, and the RNA signal is shown in the Fl images. Bar, 10 μm.