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Comparison of katanin and γ-tubulin localization at spindle poles in Xenopus A6 cells. Xenopus A6 cells were fixed and stained with a p60 katanin antibody (A, B, and C) and a γ-tubulin antibody (D, E, and F). Metaphase cells were identified by DAPI staining. Three examples (A and D, B and E, and C and F) of spindle poles are shown. In each case, p60 occupies a large hollow area while γ-tubulin occupies a smaller area within the p60 staining area. Bar, 2.5 μm.
