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. 1998 Jul;9(7):1847–1861. doi: 10.1091/mbc.9.7.1847

Figure 2.

Figure 2

p60 katanin is ubiquitous in vertebrate cells and tissues. Total SDS-soluble proteins (lanes 1–9) from a variety of cells and tissues were subjected to immunoblot analysis with the human p60 katanin antibody. Lane 1, MSU1.1, human fibroblast; lane 2, Hela; lane 3, A6, Xenopus; lane 4, N2A, mouse neuroblastoma; lane 5, PtK-1, marsupial; lane 6, DT40, chicken B cell; lane 7, Fox NY, mouse myeloma; lane 8, CSF Xenopus egg extract; and lane 9, adult mouse brain. Mouse brains were homogenized in the presence of Triton X-100 and subjected to centrifugation. The Triton-soluble supernatant was analyzed in lane 11. The Triton-insoluble material was extracted with SDS, and the SDS-soluble fraction was analyzed in lane 10. Note that the higher molecular weight species in brain is enriched in the Triton-insoluble, SDS-soluble fraction (lane 10).