Figure 4.

p60 and p80 katanin are coimmunoprecipitated from Xenopus extracts. (A) Immunoblot analysis of supernatants from immunoprecipitations probed with the human p60 antibody (lanes 1–5) and the human p80 antibody (lanes 6–10). Lanes 1 and 6, mock immunoprecipitations with control IgG; lanes 2 and 7, immunoprecipitation with 5 μg of p80 antibody; lanes 3 and 8, immunoprecipitation with 1 μg of p80 antibody; lanes 4 and 9, immunoprecipitation with 5 μg of p60 antibody; and lanes 5 and 10, immunoprecipitation with 1 μg of p60 antibody. (B) Analysis of pellets from immunoprecipitations with control IgG (lanes 1, 3, 5, 7, and 9) or with the human p60 antibody (lanes 2, 4, 6, 8, and 10). SDS-PAGE–resolved proteins were analyzed by silver staining (lanes 1 and 2) or Coomassie blue staining (lanes 3 and 4) or by immunoblots probed with the human p80 antibody (lanes 5 and 6) or with either of two Xenopus p80 anti-peptide antibodies (lanes 7–10).