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Quantitative analysis of antibody inhibition and immunodepletion of microtubule-severing activity in a Xenopus extract. The maximal rates of increase in microtubule number (e.g., Figure 5B, a, between 40 and 70 s) were taken from multiple reactions performed under the same conditions and averaged. Comparison of these average rates of severing is shown. Depleted samples are supernatants from immunoprecipitations with the indicated antibody.
