Figure 7.

Immunolocalization of p60 katanin in interphase fibroblasts. MSU1.1 cells were fixed and stained with a human p60 katanin antibody (A, D, and G), a β-tubulin antibody (B), a γ-tubulin antibody (E and H), and DAPI (C, F, and I). A focus of p60 staining was observed at the center of an aster of microtubules visualized with the β-tubulin antibody (A, B, and C). This focus of p60 staining colocalized with γ-tubulin (D, E, and F) even after microtubules were depolymerized with nocodazole (G, H, and I). Bar, 5 μm.