FIG. 4.

Effect of bafilomycin in HRV infection. (A and B) SDS-PAGE of radiolabeled HeLa cell lysates collected 9 h postinfection with HRV2, -3, -14, and -16 in the absence (−) or presence of 200 nM bafilomycin (+Baf.). (C) Cell lysates harvested 22 h postinfection. Lines corresponding to lysates from uninfected cells are labeled C. HeLa cells were infected with virus multiplicities of 10 (A) and 100 PFU/cell (B and C) as described in Materials and Methods. Asterisks indicate the viral capsid protein VP3, quantified for determination of virus infectivity (Table 1). Mobility and mass (kDa) of molecular size markers are indicated on the left side.