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. 2003 Nov;77(22):12184–12192. doi: 10.1128/JVI.77.22.12184-12192.2003

FIG. 3.

FIG. 3.

Analysis of a fused N terminus and an unmodified C terminus of NSP5 for VLS formation. MA104 cells were transfected with NSP5-containing plasmids, fixed, and immunostained with anti-NSP5 antibody. (A) Pattern of localization of native form of NSP5. (B) Pattern of expression of HA-NSP5. Note that this construct, in which the N terminus of NSP5 is fused, is able to form VLS. (C) Lack of VLS-forming ability of HA-NSP5-GFP, in which both the N and the C termini are fused to a nonrotavirus protein. Bars, 10 μm.