TABLE 1.

Primers used in this study

Vector	Fusion	Gene amplified	Primer
			Designation	Sequence
pEGFP-C2 (EcoRI-PstI cloning)	GFP-NSP5	ORF	5′-5254	5′-CGCGAATTCATGTCTCTCAGTATTGACGTG
			3′-5255	5′-GGCCTGCAGTTACAAATCTTCAATCAA
		NΔ65	5′-5647	5′-CGCGAATTCGCTTCAAACAATCCA
			3′-5255	5′-GGCCTGCAGTTACAAATCTTCAATCAA
		NΔ130	5′-6498	5′-CGCGAATTCGATACTAAAAAGGAG
			3′-5255	5′-GGCCTGCAGTTACAAATCTTCAATCAA
		NCΔ65	5′-5647	5′-CGCGAATTCGCTTCAAACAATCCA
			3′-6499	5′-GGCCTGCAGTTAATCCGTAGATATTGA
		CΔ66	5′-5254	5′-CGCGAATTCATGTCTCTCAGTATTGACGTG
			3′-6499	5′-GGCCTGCAGTTAATCCGTAGATATTGA
		CΔ130	5′-5254	5′-CGCGAATTCATGTCTCTCAGTATTGACGTG
			3′-8923	5′-GGCCTGCAGAGAATCAGATGGTCC
pEGFP-N3 (EcoRI-BamHI cloning)	NSP5-GFP	ORF	5′-5871	5′-CGCTCGAATTCTATGTCTCTCAGCATTGAC
			3′-5872	5′-GATGGATCCCAAATCTTCTATCAATTG
		NΔ65	5′-8250	5′-CGCTCGAATTCTGCTTCAAACGAT
			3′-5872	5′-GATGGATCCCAAATCTTCTATCAATTG
		NΔ130	5′-8251	5′-CGCTCGAATTCTGATACTAAAAAG
			3′-5872	5′-GATGGATCCCAAATCTTCTATCAATTG
		CΔ66	5′-5871	5′-CGCTCGAATTCTATGTCTCTCAGCATTGAC
			3′-8252	5′-GATGGATCCATCCGTAGATATTGA