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. Author manuscript; available in PMC: 2008 Sep 18.
Published in final edited form as: J Am Chem Soc. 2007 Aug 10;129(35):10732–10740. doi: 10.1021/ja071199j

Figure 8.

Figure 8

Circular Dichroism spectroscopy in the near-UV region. (a) The spectra of apo-PASC (25 µM protein, shown in circles) and holo-PASC (25 µM protein + 50 µM FeDPP(III), shown in squares) at 25°C demonstrate the increase in helicity upon addition of FeDPP(III). (b) Spectra of apo-PATET (50 µM protein, shown in triangles) and holo-PATET (50 µM protein + 25 µM FeDPP(III), shown in diamonds). (c) Fraction folded for PATET (apo, holo) and PASC (apo, holo) demonstrate that the Tm’s (°C) for PASC are higher than those for PATET, indicating increased stability.