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(A) Reaction scheme for the transfer of 3H labeled acetyl group to UDP-4-amino and onto Und-P by the enzymes PglD and PglC. PglA adds GalNAc to Und-PP-[3H]Bac. Aliquots of the reaction were taken over time and the organic phase subjected to liquid scintillation counting. (B) Plot of the liquid scintillation counts. Reactants included UDP-GalNAc [3H] AcCoA, Und-P, and UDP-4-amino (▲). Control reactions omitted Und-P (○) or UDP-4-amino (□).
