. Author manuscript; available in PMC: 2008 Sep 18.

Published in final edited form as: Biochemistry. 2006 Nov 14;45(45):13659–13669. doi: 10.1021/bi061456h

Table 3.

Kinetic parameters for GST-PglF and PglD compared with published values of PglE and Gne.

Enzyme	Substrate	Protein	K_m(app)	k_cat	k_cat/K_m
		pmol/assay	MM	Min⁻¹	min⁻¹x mM⁻¹

GST-PglF^a	UDP-GlcNAc	20.7	7.00	7.1	1.0
PglD^a	UDP-4-amino	0.011	0.41	4.83 × 10⁵	1.18 × 10⁶
PglE^b	UDP-4-keto	3.32	0.048	144	3000
MalE-Gne^c	UDP-GlcNAc	0.083	1.087	4938	4543
PseB^d	UDP-GlcNAc	25.8	0.05	1.5	30.6

This study, values are the average of two experiments, assays conducted at 37 ºC, pH 7.7.

From C. jejuni NCTC 11168, assays conducted at 37 ºC, pH 7.2 (18).

From C. jejuni NCTC 11168, assays conducted at 37 ºC, pH 8.0 (31).

From C. jejuni subspecies doylei ATCC49349, assays conducted at 42 ºC, pH 7.0 (33).