FIG. 1.

Analysis of RNA and DNA from Peromyscus cells infected with PCMV. (A) Demonstration of a presence of both genomic and spliced version of P33 gene in infected cells. Lanes: 1, P33-specific RT-PCR with RNA from PCMV-infected cells as a template; 2, P33-specific PCR with purified genomic PCMV DNA as a template; M, 100-bp ladder (Gibco-BRL). (B) Genomic arrangement of coding region of P33. The first exon, encoding the first 11 amino acids, is separated by an 85-bp intron from the second exon encoding an additional 404 amino acids.