FIG. 4.

Southern blot of recombinant and wt-PCMV DNA demonstrating insertion of SNV-G1 into P33. (A) PCR amplification with P33-specific primers with wt-PCMV DNA (lane 1), recombinant PCMV DNA (before FACS) (lane 2), and recombinant DNA (after FACS) (lane 3). (B) Southern blot of total DNA isolated from PMEC infected with PCMV(ΔP33:G1EGFP) (lane 1), wt-PCMV (lane 2), or uninfected control cells (lane 3). The DNAs were cleaved with BamHI, and the blot was probed with the 1.2-kb AgeI fragment containing the P33 gene. (C) Agarose gel, used for the Southern blot presented in panel B. Lane labeling is the same in panels B and C.