Figure 5.

Formation of a filamentous network by expression of a wild-type CMP in cell cultures. A–D were reacted with a mAb against CMP (1H1); E–H were reacted with a mAb against the myc tag in recombinant CMP (9E10); I was reacted with a mAb against type II collagen (II-II6B3); J–L were reacted with a mAb against type I collagen (IBA1). Bar, 40 μm. (A, E, and I) CECs. (B, F, and J) CEFs. (C, G, and K) CEFs transfected with a wild-type CMP transgene. (D, H, and L) Wild-type CMP-transfected CEF culture after testicular hyaluronidase treatment.