Figure 6.

The A1 and A2 domains of CMP are necessary for the formation of the filamentous network. (A) Constructs of domain-deleted CMP mutants in retrovirus. (1) CMP: myc-tagged wild-type CMP; (2) CMPΔA1: myc-tagged CMP in which the first A domain is deleted; (3) CMPΔA2: myc-tagged CMP in which the second A domain is deleted. The mAbs detecting recombinant CMPs and the positions of their epitopes are indicated. (B) Micrographs of immunofluorescent analysis of transfected fibroblast cultures with a mAb against the myc tag, 9E10. (A) CMP-RCAS transfected; (B) CMPΔA1-RCAS transfected; (C) CMPΔA2-RCAS transfected; (D) CMP-RCAN transfected. (C) Expression of CMP transgenes by CEFs. Western blot of conditioned medium from cultures of transfected CEFs. Seven and a half microliters of conditioned medium were loaded in each lane. Reducing conditions were indicated. The antibody used to detect CMP was 1H1.