FIG. 3.
Fusion of 89.6PI variants with a triple substitution CCR5 mutant. R5 variants (A) and R5X4 variants (B) from the 89.6PI swarm were tested for the ability to fuse with a CCR5 substitution mutant (11/197/276) containing changes in the N-terminal (D11A), second extracellular loop (K197A), and third extracellular loop (D276A) regions. Data indicate luciferase reporter gene expression as a percentage of that measured with wild-type CCR5 for each env gene and represent means ± standard error of the mean for three independent experiments. For clarity, R5X4 Envs that showed fusion ≥50% of that seen with wild-type CCR5 when tested with the substitution mutant are underlined.