FIG. 6.

Action of dicer on full-length and 66-nt hairpin forms of HDV and other RNAs. Seven different species of ³²P-labeled RNA were analyzed on gels of 15% acrylamide-7 M urea following incubation without (−) or with (+) dicer. The RNAs used were as follows: lanes 1 and 2, 100% double-strand (ds) RNA; lanes 3 and 4, unit-length genomic HDV RNA; lanes 5 and 6, unit-length modified HDV genomic RNA; lanes 7 and 8, 66-nt miR-122 precursor; lanes 9 and 10, 66-nt HDV genomic hairpin; lanes 11 and 12, 66-nt PSTVd RNA hairpin; lanes 13 and 14, 66-nt ASBVd RNA hairpin. After electrophoresis, the RNAs were electrotransferred to a nylon membrane, after which ³²P was quantitated by use of a bioimager. Size markers p and q were as described for Fig. 1.