Fig. 4.

Fas-sufficient canonical HKI65 B cells do not participate in the Ars-induced IgG AFC response in Fas-deficient hosts. Splenocytes from HKI65 mice were adoptively transferred into B6 or B6.lpr mice and the resulting chimeric mice immunized 12 h later with 100 μg of Ars–KLH in alum. Mice were sacrificed on day 6 after immunization and the E4 clonotype-specific immune response was assessed using ELISpot analysis of splenocytes. ELISpot membranes were coated with either anti-mouse IgG (gamma chain specific) or anti-mouse IgM (mu chain specific) and ELISpots developed with the E4 mAb. Data from individual mice are indicated by open and filled circles. P values for comparisons of data from chimeras generated using Fas-sufficient and -deficient hosts, calculated using the Student’s t-test, are shown.