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ER-targeted protein A (Ptn A) chimeras maintain increased FHV RNA accumulation in vivo after 72 h of induction. Total RNA from equivalent numbers of cells was separated by electrophoresis and blotted with a 32P-labeled complementary riboprobe that detected (+)RNA1 and (+)RNA3. Duplicate representative samples from yeast cells expressing pF1fs plus pFA, pFA-HCV, or pFA-P450 are shown.
