FIG. 3.

Interaction of the N protein with fibrillarin in vivo determined by the dual-luciferase reporter assay in the mammalian two-hybrid system. HeLa cells were cotransfected with pM-N and pVP-fibrillarin, along with the reporter plasmid p5xGAL4SV40-luc, in a ratio of 5:5:1. The cells were harvested 48 h posttransfection, and luciferase activities were measured. The data represent the means of three independent experiments plus 1 standard deviation after normalization using Renilla luciferase expression as an internal control.