FIG. 8.
Binding of the N protein to rRNA. Either the GST-N fusion protein expressed in E. coli or the purified PRRS virions (A, lanes 4 and 5; B, lanes 3 and 6) was resolved by SDS-PAGE and transferred to membranes. The membranes were hybridized with the total cellular RNA (A) or with either 28S or 18S rRNA (B) extracted from uninfected Marc-145 cells. The RNAs were radiolabeled in vitro using [γ-32P]ATP and polynucleotide kinase. N-IP, N immunoprecipitated using MAb SDOW17; GST-VP8, GST fused with rotavirus VP8 protein.