Fig. 3.

Morphodynamics and cytosketetal changes of cells transfected with the indicated GEF and GAP siRNAs. (A) Time-lapse series of control and ArhGAP30 siRNA transfected neurons. Neurite tip trajectory is shown in red. (Scale bar, 50 μm.) (B) Representative neurite tip tracks of control, ArhGAP30, Dock4, and Dock10 siRNA transfected cells. (Scale bar, 100 μm.) (C) Control, SrGAP2, and Trio siRNA transfected cells were allowed to spread for 8 h on laminin-coated coverslips and immunostained for actin (green), tubulin (red), and nucleus (blue). (D) Fluorescence micrographs of growth cone morphologies associated with BCR and SrGAP2 knockdowns. Cells were immunostained for actin (green) and tubulin (red). White arrows indicate high-density arrays of filopodia. The bar graph shows the occurrence of growth cones with “low” or “high” filopodia density from neurites longer than one soma length scored on four different regions of the coverslip from a 24-h time point. P values between control and specific knockdowns were computed by using a t test. (E) Time-lapse analysis of neurite formation of SrGAP2 and Trio knockdown cells. Red arrows indicate phase refractile globular structures indicative of retracting neurites. (Scale bar, 20 μm.)