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. 1998 Jul;9(7):1873–1889. doi: 10.1091/mbc.9.7.1873

Figure 5.

Figure 5

Expression of a mutant fimbrin (Sac6p) with an altered actin-binding domain suppresses the invasion and cell elongation defects of an act1–120/act1–120 diploid. Strains BCY209 (ACT1/ACT1), BCY210 (act1–120/act1–120), BCY372 (sac6/sac6), BCY412 (act1–120/act1–120 〈SAC6+〉), BCY411 (act1–120/act1–120 〈sac6–10〉), BCY432 (act1–111/act1–111 〈sac6–10〉), and BCY433 (act1–124/act1–124 〈sac6–10〉), where genotypes in angle brackets refer to relevant genotypes of plasmids AAB117 (SAC6 URA3 CEN) and AAB289 (sac6–10 URA3 CEN) (Brower et al., 1995), were streaked to SLAD agar and incubated at 30°C for 4 d. The resultant colonies were photographed before and after washing the agar surface. Long cells were quantified as described in MATERIALS AND METHODS and are reported with SE. Bar, 500 μm.