The actin cytoskeleton in actin mutants grown
under conditions that induce PH growth. Shown are wild-type and actin
mutant strains grown in SLAD alginate and stained with
rhodamine–phalloidin (to detect F-actin) and DAPI (to detect DNA)
as described in MATERIALS AND METHODS. F-actin images are in stereo.
Strains shown are BCY209 (ACT1/ACT1), BCY157
(act1–110/ACT1), BCY204
(act1–111/act1–111), BCY202
(act1–112/act1–112), BCY210
(act1–120/act1–120), and BCY168
(act1–131/ACT1). Bar, 10 μm.