Table 2.
Summary of growth phenotypes of actin mutants on YPD
| Allele | Growth of homozygote at 30°C | Growth of homozygote at 36°C | Dominance |
|---|---|---|---|
| WT | +++ | ++ | |
| 104 | +++ | ++ | |
| 109 | ND | ND | Recessive |
| 110 | ND | ND | Dominant |
| 111 | ++ | − | Recessive |
| 112 | +++ | +/− | Dominant |
| 113 | +++ | +/− | Recessive |
| 117 | +++ | ++ | |
| 120 | +++ | − | Recessive |
| 124 | +++ | +/− | Recessive |
| 129 | +++ | +/− | Recessive |
| 131 | ND | ND | Dominant |
| 132 | + | − | Recessive |
WT, wild-type; ND, not determined, as these mutations are recessive lethal. Growth phenotypes were determined by spotting serial dilutions of a saturated culture and assessing plating efficiency and size of colonies after 3 d growth on YPD agar plates. Dominance refers specifically to effect of heterozygous mutation on growth on YPD at 36°C.