Table 5.
Allele | WT | Δ | 104 | 111 | 112 | 113 | 117 | 120 | 124 | 129 | 132 |
---|---|---|---|---|---|---|---|---|---|---|---|
WT | +++ | ++ | +++ | +++ | − | +++ | +++ | +++ | ++ | + | + |
104 | + | + | − | ++ | + | +++ | + | − | − | ||
111 | − | −a | +++ | ++ | +++ | − | − | − | |||
112 | − | − | − | − | − | − | − | ||||
113 | +++ | +++ | +++ | ++ | + | + | |||||
117 | ++ | +++ | + | − | + | ||||||
120 | − | + | + | − | |||||||
124 | − | − | − | ||||||||
129 | − | − | |||||||||
132 | − |
Filamentation of heteroallelic act1/act1 diploids was scored after 4 d growth at 30°C on SLAD agar plates as described in MATERIALS AND METHODS. WT, wild type.
Very poor growth of heteroallelic strain at 30°C (see Figure 4).