FIG. 8.

Effects of lyases (heparitinase and chondroitinase) on HIV-1 binding, entry, and replication in primary human BMVECs. Effects of lyases on HIV-1 YU-2 binding (4°C) (A), internalization (37°C) (B), and replication (C) in BMVECs and HIV-1 NL4-3 binding (4°C) (D), internalization (37°C) (E), and replication (F) in BMVECs. Cells were treated according to standard procedures, as described in Materials and Methods. The graphs represent a single experiment (samples were analyzed in duplicate). C, BMVECs pretreated with 5 U of chondroitinase per ml; H, BMVECs pretreated with 5 U of heparitinase per ml; C+H, BMVECs pretreated with a combination of 5 U of chondroitinase per ml and 5 U of heparitinase per ml; −Enzyme, untreated BMVECs; −Enzyme/−Virus, untreated and uninfected BMVECs.