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. Author manuscript; available in PMC: 2009 Sep 15.
Published in final edited form as: Adv Drug Deliv Rev. 2008 Apr 24;60(12):1383–1388. doi: 10.1016/j.addr.2008.04.010

Figure 7.

Figure 7

Selection of amino-acid residues for mutation [iv]. The crystal structure of the CHA255-ligand complex from reference [iii] was modified to simulate an acryloyl substituent at the para-position of the aromatic ring of the ligand. Residues 95 and 96 of the antibody light chain (Kabat numbering 93 and 94) showed side chains that were not in van der Waals contact with the ligand, yet were only a few Ångstroms away from the para-substituent. Reproduced from A.J. Chmura, M.S. Orton, C.F. Meares, Antibodies with infinite affinity, Proc. Natl. Acad. Sci. USA 98 (2001) 8480-8484.