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. 2008 Oct;173(4):927–937. doi: 10.2353/ajpath.2008.071149

Figure 7.

Figure 7

Expression of Glomerular Basement Membrane Components in Cd151-Null Kidneys. Immunofluorescence analysis of GBM laminin chains (A–H), nidogen (I, J), and collagen type IV α1–5 chains (K–T) in 3-week old Cd151+/+ and Cd151−/− kidneys. In wild-type kidneys at this age, the laminin network is composed of the α5, β2, and γ1 laminin chains (ie, laminin 521) (A, E, G) while the β1 chain of laminin has switched off in the GBM and is restricted to the mesangial matrix (C). In the Cd151 knock-out kidneys however there is persistence of the laminin β1 chain in the GBM (D) together with expression of α5, β2, and γ1 laminin chains. All four chains of laminin also show increased expression specifically in the GBM, as does nidogen/entactin (I–J). In both 3-week-old wild-type and Cd151−/− kidneys, the switch from the α1α1α2 collagen network to the α3α4α5 network has normally occurred (K–T). However there is a strong increase in expression of the α4 and α5 chains of collagen type IV in the thickened GBM of Cd151−/− kidneys (R, T). The representative results from two mice in each group are shown. Original magnification: ×400.