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. 2008 Oct;173(4):962–972. doi: 10.2353/ajpath.2008.080358

Figure 1.

Figure 1

FADD-DN mice are protected from apoptotic, but not necrotic liver injury. A: WT and FADD-DN mice were treated with ConA/GalN (left panel) or ConA alone (right panel) and sacrificed at 8 hours later. The liver cytosol was analyzed for caspase-3, -8, and -9 activities using the corresponding substrates. Results (mean ± SD) are expressed as the fold of changes over the non-treated control. Groups that had significantly different responses are indicated (t-test; *P < 0.05; **P < 0.01). B: The liver cytosols of mice treated with ConA/GalN for different times were analyzed by immunoblot (cyto c, p15 cytochrome c, C-9: p49 pro-caspase-9 and p39 cleaved caspase-9, C-3: p20 and p19 cleaved caspase-3, and p37 GAPDH). C: Serum ALT levels (mean ± SD) from ConA/GalN or ConA only-treated mice (6 or 8 hours, respectively). D: Liver sections of WT (a–c) and FADD-DN (d–f) mice treated with ConA/GalN (b, e) or ConA alone (c, f), or left untreated (a, d) for 8 hours were H&E stained and representative images were shown. Original magnification: ×100.