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C5aR siRNA down-regulates TNF-α, MIP-2 and KC expression. 48 h after gene silencing, kidneys (n = 3, per group) were clamped for I/R injury. 24 h after reperfusion, kidney tissues were harvested and used to extract total RNA. Expression of TNF-α was determined by real time PCR (A). Expression of MIP-2 (B) and KC (C) was detected by semiquantitative PCR. Statistical significance as compared with controls was denoted at (∗) P < 0.05; (∗∗) P < 0.01.
