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. 2008 Oct;173(4):1029–1041. doi: 10.2353/ajpath.2008.071062

Figure 5.

Figure 5

Representative examples of cerebellar neuropathology during the initial stages of EAE in Prnp+/+ and Prnp−/− animals. (A) Marked perivascular infiltration was observed at 12 dpi only in Prnp−/− animals. Infiltrating cells were mostly Iba-1 positive and were more abundant than CD3ε-positive cells in both Prnp+/+ and Prnp−/− animals at 12 dpi. (B) Analysis of mean pro-inflammatory molecule mRNA relative fold-change (±SEM) in lumbar spinal cords of healthy and EAE Prnp+/+ (open bars) and Prnp−/− (filled bars) animals. Real-time RT-PCR showed that mRNA levels for IFN-γ, TNF-α, IL-1β, iNOS, and RANTES were significantly higher in Prnp−/− EAE at 12 dpi relative to Prnp+/+ EAE, when expressed as fold-increase above expression seen in healthy Prnp+/+ animals. IL-17A did not show significant difference between Prnp+/+ and Prnp−/− EAE at this time point. Basal mRNA levels for each gene did not differ between Prnp−/− and Prnp+/+ animals. All real-time experiments were done in duplicate for each RNA sample (n = three per group, *P < 0.05; **P < 0.01, ***P < 0.001; ANOVA, Tukey’s multiple comparison test).