Figure 1.

Detection of citrullinated proteins in brains from control and ME7 scrapie-infected mice. Whole brains and various brain sections of control (lanes 1 to 3) and ME7-infected mice (lanes 4 to 6) were homogenized, and then citrullinated proteins were detected by Western blot analysis using a polyclonal anti-modified citrulline antibody. Note that citrullinated proteins were increased in scrapie-infected regions compared to controls. Each lane shows the results for a homogenate obtained from brains of each individual mouse. Asterisks indicate the citrullinated proteins. These results are representative of at least three separate experiments.