Figure 7.

Spatial distribution of SMA on day 9. A, B: Laser scanning microscopy (A) of double-immunostaining [CD31-red (endothelium); SMA-green (SMA-positive cells)] and light microscopic inspection of toluidine-blue-stained consecutive sections (B–B‴) revealed that SMA-positive cells are located in the vicinity of pillars (arrows in A and B) and also as peri-endothelial covering (arrowheads in A and B). The double arrows in A and B indicate transluminar tissue pillars. C: Estimation of SMA by immunoblotting revealed higher levels in the medullary than in the cortical tumor regions. (C = cortex, M = medulla).