Table 1.
Primary Antibodies Used in This Study
| Specificity | Designation | Working concentration | Specification | Source |
|---|---|---|---|---|
| IL-33 | IL-33 Nter | 1.8 μg/ml | Rabbit | Eurogentec |
| IL-33 | Nessy-1 | 1 μg/ml | Mouse IgG1 | Alexis |
| IL-33 | ALX-210-447 | 1 μg/ml | Rabbit | Alexis |
| IL-33 | ALX-210-993 | 1 μg/ml | Rabbit | Alexis |
| CD31 | HEC7 | 1 μg/ml | Mouse IgG2a | Fitzgerald |
| CD31 | RB-10333 | 0.4 μg/ml | Rabbit | LabVision |
| PCNA | PC10 | 1.25 μg/ml | Mouse IgG2a | DAKO |
| Phospho-Rb (Ser807/811) | #9308 | 1:100 | Rabbit | Cell signaling |
| Ki67 | MIB-1 | 10 μg/ml | Mouse IgG1 | DAKO |
| Ki67 | ab15580 | 5 μg/ml | Rabbit | Abcam |
| Lymphatic EC | D2-40 | 0.26 μg/ml | Mouse IgG1 | DAKO |
| VE-cadherin | 4.8.G | 1:1000 | Mouse IgG1 | O. Bakke |
| VE-cadherin | BV9 | 10 μg/ml | Mouse IgG2a | Hycult biotechnology |
| Actin | sc-8432 | 1:500 | Mouse IgG1 | Santa Cruz |
| Histone H1 | sc-10806 | 1 μg/ml | Rabbit | Santa Cruz |
| Irrelevant control | H0892 | Matched | Rabbit | Sigma |
| Irrelevant control | MOPC 21 | Matched | Mouse IgG1 | Sigma |