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Mass spectra of cytochrome C in 99.9:0.1 (v:v) water:acetic acid (240 pmol total, 2.4 pmol nozzle-1) obtained by AMUSE ionization from a chip with 3-μm nozzles and using either (A) nitrogen at ambient temperature or (B) nitrogen heated to 40 °C to operate the Venturi device. Operating conditions: 60 μL min-1 liquid flow rate, 100 VDC, 8 L min-1, QiT detection.
