Figure 3.

Cell growth and expression of RanBP1 proteins in S. pombe. S. pombe cells transformed with the full-strength (nmt, filled and empty circles), medium-strength (nmt*, filled and empty triangles), or low-strength (nmt**, filled and empty squares) nmt promoter plasmids expressing full-length sbp1p (A), RBD (Sp) (B), RanBP1 (m) (C), or RBD (m) (D) were grown in Edinburgh minimal medium in the presence of 5 μg/ml thiamine (+, filled symbols) or in its absence (−, empty symbols). The optical density at 600 nm was monitored as a function of time. Ten micrograms of protein extracts from cells grown to midlog phase in the presence of thiamine or for an equal amount of time in its absence were analyzed by immunoblotting using mAbs against the T7 or HA epitope tags. Arrows indicate full-length proteins.