Figure 5.

Nuclear protein import of GST-GFP-NLS in S. pombe expressing sbp1p, RanBP1 (m), or their RBDs. S. pombe cells were cotransformed with a GST-GFP-NLS–expressing construct and with nmt promoter plasmids expressing the indicated RanBP1 or RBD proteins (nmt, full-; nmt*, medium-; and nmt**, low-strength promoter). Cells were grown in the absence of thiamine and fixed by methanol, and the NLS protein was visualized by fluorescence microscopy. Vector indicates control transformations using nmt plasmids lacking RanBP1 or RBD inserts.