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NLS nuclear protein import in rescued S. pombe sbp1-ΔC genomic mutant cells. sbp1-ΔC mutant cells rescued with the indicated nmt* constructs were transformed with a GST-GFP-NLS expressing construct. Cells were grown to midlog phase in the absence of thiamine or for an equal amount of time in its presence, fixed in methanol, and stained with DAPI before mounting. The GFP signal was analyzed by direct fluorescence microscopy.
